Title: | Raw and Processed Matrices of the PBMC 3k Dataset |
---|---|
Description: | What the package does (one paragraph). |
Authors: | Paul Hoffman [aut, cre] , 10x Genomics, Inc. [cph] |
Maintainer: | Paul Hoffman <[email protected]> |
License: | CC BY 4.0 |
Version: | 0.1.0 |
Built: | 2024-11-15 03:20:52 UTC |
Source: | https://github.com/mojaveazure/pbmc3k |
2,700 peripheral blood mononuclear cells (PBMC) from 10X genomics; this is
effectively what one would get with
Seurat::Read10X()
pbmc3k
pbmc3k
A dgCMatrix
with the raw,
unfiltered PBMC 3k counts
https://support.10xgenomics.com/single-cell-gene-expression/datasets/1.1.0/pbmc3k
Log-Normalized PBMC 3k: SingleCellExperiment Edition
pbmc3k.sce.logcounts
pbmc3k.sce.logcounts
A dgCMatrix
with the
log-normalized PBMC 3k counts
There is not a raw, filtered matrix following a Bioconductor workflow
as scuttle does not filter the raw counts matrix in the same way that
SeuratObject::CreateSeuratObject()
does
https://support.10xgenomics.com/single-cell-gene-expression/datasets/1.1.0/pbmc3k
Orchestrating Single-Cell Analysis with Bioconductor version 1.8.0
scuttle::perCellQCMetrics()
,
scuttle::quickPerCellQC()
,
scuttle::perCellQCFilters()
,
scuttle::isOutlier()
Log-normalized matrices:
pbmc3k.seurat.norm
Raw PBMC 3k Counts: Seurat Edition
pbmc3k.seurat.counts
pbmc3k.seurat.counts
A dgCMatrix
with the raw,
filtered PBMC 3k counts
https://support.10xgenomics.com/single-cell-gene-expression/datasets/1.1.0/pbmc3k
Seurat PBMC 3k Guided Clustering Tutorial
SeuratObject::CreateSeuratObject()
,
SeuratObject::CreateAssayObject()
Seurat-processed matrices:
pbmc3k.seurat.norm
Log-Normalized PBMC 3k: Seurat Edition
pbmc3k.seurat.norm
pbmc3k.seurat.norm
A dgCMatrix
with the
log-normalized PBMC 3k counts
https://support.10xgenomics.com/single-cell-gene-expression/datasets/1.1.0/pbmc3k
Seurat PBMC 3k Guided Clustering Tutorial
Seurat-processed matrices:
pbmc3k.seurat.counts
Log-normalized matrices:
pbmc3k.sce.logcounts